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plk1 fret sensor c jun substrate plasmid40  (Addgene inc)


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    Structured Review

    Addgene inc plk1 fret sensor c jun substrate plasmid40
    Plk1 Fret Sensor C Jun Substrate Plasmid40, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plk1+fret+sensor+c+jun+substrate+plasmid40/Plk1+FRET+sensor+c-jun+substrate+(Plasmid+%2345203)/pm37773176-211-159-164
    Average 93 stars, based on 4 article reviews
    plk1 fret sensor c jun substrate plasmid40 - by Bioz Stars, 2026-10
    93/100 stars

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    Cell Culture:

    Article Title: Mild replication stress causes premature centriole disengagement via a sub-critical Plk1 activity under the control of ATR-Chk1.
    Article Snippet: .. Cell culture and drug treatments hTERT-RPE1 (ATCC: CRL-4000) and hTERT-RPE1 EB3-eGFP/H2BmCherry cells (kind gift by Willy Krek, ETH Zurich, Switzerland) were cultured in Dulbecco’s Modified Eagle’s Medium (Thermo Fisher Scientific: 61965-026) supplemented with 10% fetal calf serum (FCS) (Biowest: S181S-500) and 100 U/ml of each penicillin and streptomycin (P/S) (Thermo Fisher Scientific: 15140122) at 37 °C, 95% relative humidity and 5% CO2 in humidified CO2 incubator. hTERT-RPE1 FRT/TR (kind gift from Johnathan Pines, Institute of Cancer Research, United Kingdom), hTERT-RPE1 OSTR1 cells, hTERT-RPE1 Cyclin-A2-double degron cells and hTERT-RPE1 CyclinB1-double degron cells (all kind gifts by Helfrid Hochegger, University of Sussex, United Kingdom) were cultured in Dulbecco’s Modified Eagle’s Medium (Thermo Fisher Scientific: 61965-026) supplemented with 10% tetracycline free fetal calf serum (FCS) (Biowest: S181T-500) and 100 U/ml of each penicillin and streptomycin (P/S) (Thermo Fisher Scientific: 15140122) at 37 °C, 95% relative humidity and 5% CO2 in humidified CO2 incubator. hTERT-RPE1 Plk1 FRET Sensor cells were prepared by transfecting Plk1-FRET sensor c-jun substrate plasmid40 (Addgene: 45203) in hTERT-RPE1 cells using X-tremeGENETM 9 (Merck: XTG9-RO) transfection reagent according to manufacturer’s instructions. .. Transfected cells were selected with 600 μg/ml of G418 (Invivogen: ant-gn-5) in DMEMwith 10% FCS and 100 U/ml P/S, followed by single cell cloning. hTERTRPE1: Myc-Separase (RNAi-resistant) cells were prepared by transfecting hTERT-RPE1 FRT/TR cells with 0.5 μgMyc-Separase (WT) and 4.5 μg pOG44 plasmids using X-tremeGENETM 9 (Merck: XTG9-RO) transfection reagent according to manufacturer’s instructions.

    Modification:

    Article Title: Mild replication stress causes premature centriole disengagement via a sub-critical Plk1 activity under the control of ATR-Chk1.
    Article Snippet: .. Cell culture and drug treatments hTERT-RPE1 (ATCC: CRL-4000) and hTERT-RPE1 EB3-eGFP/H2BmCherry cells (kind gift by Willy Krek, ETH Zurich, Switzerland) were cultured in Dulbecco’s Modified Eagle’s Medium (Thermo Fisher Scientific: 61965-026) supplemented with 10% fetal calf serum (FCS) (Biowest: S181S-500) and 100 U/ml of each penicillin and streptomycin (P/S) (Thermo Fisher Scientific: 15140122) at 37 °C, 95% relative humidity and 5% CO2 in humidified CO2 incubator. hTERT-RPE1 FRT/TR (kind gift from Johnathan Pines, Institute of Cancer Research, United Kingdom), hTERT-RPE1 OSTR1 cells, hTERT-RPE1 Cyclin-A2-double degron cells and hTERT-RPE1 CyclinB1-double degron cells (all kind gifts by Helfrid Hochegger, University of Sussex, United Kingdom) were cultured in Dulbecco’s Modified Eagle’s Medium (Thermo Fisher Scientific: 61965-026) supplemented with 10% tetracycline free fetal calf serum (FCS) (Biowest: S181T-500) and 100 U/ml of each penicillin and streptomycin (P/S) (Thermo Fisher Scientific: 15140122) at 37 °C, 95% relative humidity and 5% CO2 in humidified CO2 incubator. hTERT-RPE1 Plk1 FRET Sensor cells were prepared by transfecting Plk1-FRET sensor c-jun substrate plasmid40 (Addgene: 45203) in hTERT-RPE1 cells using X-tremeGENETM 9 (Merck: XTG9-RO) transfection reagent according to manufacturer’s instructions. .. Transfected cells were selected with 600 μg/ml of G418 (Invivogen: ant-gn-5) in DMEMwith 10% FCS and 100 U/ml P/S, followed by single cell cloning. hTERTRPE1: Myc-Separase (RNAi-resistant) cells were prepared by transfecting hTERT-RPE1 FRT/TR cells with 0.5 μgMyc-Separase (WT) and 4.5 μg pOG44 plasmids using X-tremeGENETM 9 (Merck: XTG9-RO) transfection reagent according to manufacturer’s instructions.

    Transfection:

    Article Title: Mild replication stress causes premature centriole disengagement via a sub-critical Plk1 activity under the control of ATR-Chk1.
    Article Snippet: .. Cell culture and drug treatments hTERT-RPE1 (ATCC: CRL-4000) and hTERT-RPE1 EB3-eGFP/H2BmCherry cells (kind gift by Willy Krek, ETH Zurich, Switzerland) were cultured in Dulbecco’s Modified Eagle’s Medium (Thermo Fisher Scientific: 61965-026) supplemented with 10% fetal calf serum (FCS) (Biowest: S181S-500) and 100 U/ml of each penicillin and streptomycin (P/S) (Thermo Fisher Scientific: 15140122) at 37 °C, 95% relative humidity and 5% CO2 in humidified CO2 incubator. hTERT-RPE1 FRT/TR (kind gift from Johnathan Pines, Institute of Cancer Research, United Kingdom), hTERT-RPE1 OSTR1 cells, hTERT-RPE1 Cyclin-A2-double degron cells and hTERT-RPE1 CyclinB1-double degron cells (all kind gifts by Helfrid Hochegger, University of Sussex, United Kingdom) were cultured in Dulbecco’s Modified Eagle’s Medium (Thermo Fisher Scientific: 61965-026) supplemented with 10% tetracycline free fetal calf serum (FCS) (Biowest: S181T-500) and 100 U/ml of each penicillin and streptomycin (P/S) (Thermo Fisher Scientific: 15140122) at 37 °C, 95% relative humidity and 5% CO2 in humidified CO2 incubator. hTERT-RPE1 Plk1 FRET Sensor cells were prepared by transfecting Plk1-FRET sensor c-jun substrate plasmid40 (Addgene: 45203) in hTERT-RPE1 cells using X-tremeGENETM 9 (Merck: XTG9-RO) transfection reagent according to manufacturer’s instructions. .. Transfected cells were selected with 600 μg/ml of G418 (Invivogen: ant-gn-5) in DMEMwith 10% FCS and 100 U/ml P/S, followed by single cell cloning. hTERTRPE1: Myc-Separase (RNAi-resistant) cells were prepared by transfecting hTERT-RPE1 FRT/TR cells with 0.5 μgMyc-Separase (WT) and 4.5 μg pOG44 plasmids using X-tremeGENETM 9 (Merck: XTG9-RO) transfection reagent according to manufacturer’s instructions.



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    93
    Addgene inc plk1 fret sensor c jun substrate plasmid40
    Plk1 Fret Sensor C Jun Substrate Plasmid40, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plk1+fret+sensor+c+jun+substrate+plasmid40/Plk1+FRET+sensor+c-jun+substrate+(Plasmid+%2345203)/pm37773176-211-159-164
    Average 93 stars, based on 1 article reviews
    plk1 fret sensor c jun substrate plasmid40 - by Bioz Stars, 2026-10
    93/100 stars
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